Human Endocrine Gland Derived Vascular Endothelial Growth Factor ELISA Kit

Antibodies.com supplies this sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit for the in vitro quantitative determination of human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The assay is intended for use with a colorimetric microplate reader, with absorbance read at 450 nm.

The kit employs a pre-coated 96-well microplate (12 x 8 well strips) using the sandwich enzyme immunoassay technique. Standards and samples are added to wells pre-coated with an antibody specific for EG-VEGF, where the target is captured by the immobilized antibody. Following incubation and washing, a biotinylated anti-EG-VEGF antibody is added, followed by an HRP-avidin conjugate and TMB substrate. The resulting enzymatic reaction produces a blue colour that turns yellow upon addition of the acidic stop solution; the intensity of the yellow colour is directly proportional to the amount of EG-VEGF captured in each well.

Quantitative performance is reported across an assay range of 15.6-1000 pg/ml with a sensitivity of <7.2 pg/ml and a total assay time of 4 hours 30 minutes. Validation work includes recovery testing across serum, EDTA plasma and heparin plasma, with average recoveries of 91% in serum (range 80-102%), 91% in EDTA plasma (range 81-101%) and 84% in heparin plasma (range 80-89%). Linearity has been demonstrated at sample dilutions of 1:2, 1:4, 1:8 and 1:16 in each matrix. Kit components should be stored at +4°C or -20°C as instructed and not used past the expiration date.