Human Electrogenic Sodium Bicarbonate Cotransporter 4 ELISA Kit

From Antibodies.com, this Sandwich ELISA kit is designed for the in vitro quantitative determination of human SLC4A5, also known as Electrogenic Sodium Bicarbonate Cotransporter 4, in tissue homogenates or other biological fluids. The assay employs the sandwich enzyme immunoassay technique on a pre-coated microplate, with a colourmetric readout measured at 450 nm on a microplate reader. Total assay time is 4 hours 30 minutes and reactivity is human.

Quoted performance figures from the manufacturer include a sensitivity of 0.119 ng/ml and an assay range of 0.312–20 ng/ml. Sample recovery data are reported for serum (n=5, range 80–102%, average 91%), EDTA plasma (n=5, range 81–100%, average 90%) and heparin plasma (n=5, range 80–89%, average 84%). Dilution linearity at 1:2, 1:4, 1:8 and 1:16 for serum (n=5) is given as 87–91%, 87–107%, 74–101% and 92–97%; for EDTA plasma (n=5) as 90–105%, 84–101%, 90–101% and 79–108%; and for heparin plasma (n=5) as 84–95%, 92–105%, 82–105% and 89–91%.

Supplied components include a pre-coated 96 well microplate (12 x 8 well strips), lyophilized standard (2 vials), Detection Solution A (120 μl), Detection Solution B (120 μl), 30X Wash Buffer (20 ml), Sample Dilution Buffer (45 ml), TMB Substrate (9 ml), Stop Solution (6 ml) and plate sealers (5 adhesive strips). Component storage ranges from +4°C to -20°C as indicated, and the kit should not be used past its expiration date.