Human Elastin Microfibril Interface Located Protein 2 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com is designed for the in vitro quantitative determination of human EMILIN2 in tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) and the sandwich enzyme immunoassay technique, with a biotinylated anti-EMILIN2 detection antibody, HRP-Avidin conjugate, and TMB substrate read at 450 nm.

Reported performance specifications include an assay range of 0.156–10 ng/ml, a sensitivity of <0.058 ng/ml, and a total assay time of 4h 30m. The kit is supplied with a pre-coated 96 well microplate, lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and adhesive plate sealers. Components are stored at +4°C or –20°C as instructed and should not be used past the expiration date.

Manufacturer literature provides sample recovery data for serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, 1:8, and 1:16 (n=5 per matrix).