Human Elastase 2B ELISA Kit

This sandwich ELISA from Antibodies.com is designed for the in vitro quantitative determination of human Elastase 2B in serum, plasma, tissue homogenates or other biological fluids. Listed under the Assays and Kits category, the kit uses a pre-coated 96-well microplate in a 12 x 8 well strip configuration and follows a standard sandwich enzyme immunoassay workflow with a biotinylated detection antibody, HRP-avidin conjugate and TMB substrate, read at 450 nm on a colorimetric microplate reader. The stated sensitivity is 0.107 ng/ml and the assay range is 0.312-20 ng/ml, with a total assay time of 4h 30m.

Reported average recovery is 91% in serum (range 83%-100%), 96% in EDTA plasma (90%-103%) and 94% in heparin plasma (90%-99%), across n=5 for each matrix. Dilution linearity across 1:2, 1:4, 1:8 and 1:16 is reported as 90-99%, 82-108%, 82-96% and 92-103% for serum; 84-105%, 88-94%, 89-107% and 72-106% for EDTA plasma; and 89-92%, 88-108%, 84-102% and 90-98% for heparin plasma. Components should be stored at +4°C or -20°C as instructed, and the kit should not be used past the stated expiration date.