Human Dolichyl Phosphate-N-Acetylglucosaminephosphotransferase 1 ELISA Kit

A sandwich ELISA from Antibodies.com is intended for the in vitro quantitative determination of human DPAGT1/GPT (Dolichyl Phosphate N-Acetylglucosaminephosphotransferase 1) in tissue homogenates, cell lysates or other biological fluids.

The assay employs a pre-coated microplate with a biotinylated anti-DPAGT1/GPT detection antibody, an HRP-Avidin conjugate and TMB substrate, with absorbance read at 450nm on a colorimetric microplate reader. Manufacturer-reported performance figures are a sensitivity of 0.059 ng/ml and an assay range of 0.156-10 ng/ml, with a total assay time of 4h 30m. Kit components are stored at +4°C or -20°C as instructed and must not be used past the expiration date.

Validation data reported by the manufacturer include spike-and-recovery in serum (n=5, 80-102%, average 91%), EDTA plasma (n=5, 81-101%, average 91%) and heparin plasma (n=5, 80-89%, average 84%), together with dilution linearity at 1:2, 1:4, 1:8 and 1:16 across the same sample types. The kit is suitable for use with cell lysates and tissue homogenates.