Human DNA Methyltransferase 3A ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com is designed for the in vitro quantitative determination of human DNA Methyltransferase 3A (Dnmt3a) in tissue homogenates, cell lysates, and other biological fluids. The assay employs a pre-coated 96-well microplate (12 x 8 well strips) using a biotinylated anti-Dnmt3a detection antibody and an HRP-avidin conjugate, with TMB substrate producing a colorimetric change read at 450 nm in a microplate reader.

The quantitative range is 0.156-10 ng/ml with a sensitivity of 0.059 ng/ml, and total assay time is approximately 4 hours 30 minutes. Validated sample matrices include serum (average recovery 91%, range 83-100%), EDTA plasma (average 95%, range 91-99%), and heparin plasma (average 94%, range 90-99%), with linear dilution behaviour demonstrated across 1:2 to 1:16 sample dilutions. Kit components are stored at +4°C or -20°C as indicated, and the product is intended for research use only.