Human Dihydroorotate Dehydrogenase ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) is designed for the in vitro quantitative determination of human Dihydroorotate Dehydrogenase (DHODH) in tissue homogenates, cell lysates or other biological fluids. The kit uses a pre-coated 96-well microtiter plate, a biotinylated anti-DHODH detection antibody, and an HRP-Avidin conjugate. TMB substrate produces a blue product that turns yellow upon addition of acidic stop solution; absorbance is read at 450 nm in a microplate reader.

According to the supplied documentation, the assay has a sensitivity of 0.051 ng/ml, a measuring range of 0.156-10 ng/ml, and a total assay time of 4h 30m. Kit components are stored at +4°C or -20°C as instructed and should not be used past the stated expiration date. Required equipment not included in the kit is a microplate reader capable of measuring absorbance at 450 nm ± 10 nm, a multichannel pipette reservoir or automated microplate washer, a 37°C incubator, and other standard laboratory supplies.

Precautions include avoiding skin and eye contact with the acidic Stop Solution, wearing appropriate protective equipment, and protecting TMB Substrate from prolonged light exposure. The product is intended for research use only and is not intended for diagnostic or therapeutic use.