Human Diacylglycerol-O-Acyltransferase Homolog 1 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human DGAT1 (Diacylglycerol O Acyltransferase Homolog 1) in serum, plasma, tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, a biotinylated anti-DGAT1 detection antibody, an HRP-avidin conjugate, and TMB substrate. The colourimetric reaction is read at 450 nm on a microplate reader.

Quantitative performance figures quoted by the manufacturer include an assay range of 0.312-20 ng/ml and a sensitivity of <0.114 ng/ml, with a total assay time of 4h 30m. Reported average recovery values across n=5 samples are 91% for serum (range 80%-102%), 90% for EDTA plasma (range 81%-100%), and 84% for heparin plasma (range 80%-89%).

Kit components include a pre-coated 96-well microplate, lyophilized standard, Detection Solution A (120 µl), Detection Solution B (120 µl), 30X Wash Buffer (20 ml), Sample Dilution Buffer (45 ml), TMB Substrate (9 ml), Stop Solution (6 ml), and plate sealers. Storage temperatures vary by component between +4°C and -20°C, and the kit should not be used past its expiration date.