Human Deoxycytidine Kinase ELISA Kit

Manufactured by Antibodies.com for the quantitative measurement of human Deoxycytidine Kinase (DCK), this sandwich ELISA kit is intended for use with human samples including tissue homogenates and other biological fluids. The assay uses a microplate pre-coated with an antibody specific for DCK, with bound analyte detected using a biotinylated anti-DCK antibody and an HRP-avidin conjugate. Colour development is achieved with TMB substrate, and absorbance is read at 450 nm in a microplate reader.

The reported sensitivity is 0.059 ng/ml with an assay range of 0.156–10 ng/ml, and the complete protocol can be carried out in approximately 4 hours 30 minutes. Kit components include a pre-coated microplate, lyophilised standard, Detection Solutions A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and plate sealers, to be stored at +4°C or –20°C as instructed.

In validation testing, recovery in serum samples (n=5) ranged from 80% to 102% (average 91%), in EDTA plasma from 81% to 100% (average 90%), and in heparin plasma from 80% to 89% (average 84%). Linearity across 1:2, 1:4, 1:8, and 1:16 dilutions fell within 74–108% across all three sample types. The kit is intended for research use only and is not for diagnostic or therapeutic applications.