Human Defensin Beta 103A ELISA Kit

Quantitative measurement of human hBD-3 antimicrobial peptide is enabled by the Antibodies.com Defensin Beta 103A ELISA Kit, a 48-test assay built on a 96-well microplate format. Pre-coated capture antibody, biotinylated detection reagent, recombinant standards, streptavidin-HRP conjugate, TMB substrate, and wash and diluent buffers are supplied to generate a seven-point standard curve for human Defensin Beta 103A in epithelial and immune cell research.

Each run uses 100 uL of sample per well, with serum, plasma, and cell culture supernatant as the most commonly validated matrices. The protocol supports work on keratinocyte antimicrobial responses, oral and intestinal epithelial immunity, inflammatory skin conditions, and host-pathogen interaction studies, with no significant cross-reactivity reported for related beta-defensin family members at physiological concentrations.

Reagents ship in single-use vials and should be stored refrigerated at 2 to 8 degrees C until first use, with the TMB substrate protected from light and repeated freeze-thaw cycles avoided by aliquoting working solutions. Shelf life is 12 months from manufacture when components are stored appropriately. This research-use-only kit is intended for laboratory measurement of endogenous human Defensin Beta 103A in vitro, not for diagnostic or clinical decision-making.