Human DEFB124 ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human DEFB124 in serum, plasma, tissue homogenates, and other biological fluids. The assay employs a 96-well microtiter plate pre-coated with an antibody specific for DEFB124, followed by detection with a Biotinylated Anti-DEFB124 Antibody and an HRP-Streptavidin conjugate. The enzymatic reaction is visualised using TMB substrate and measured by absorbance at 450 nm, with the concentration of DEFB124 calculated against a standard curve.

The kit has a reported sensitivity of 9.375 pg/ml and a standard curve range of 15.625–1000 pg/ml. All samples should be diluted at least 1:2 with the provided Sample Dilution Buffer before use. The entire kit should be stored at +4°C, and reagents are not to be used past the expiration date. Standards and detection reagents should be prepared shortly before use, as described in the protocol.

This product is intended for research use only and is not for diagnostic or therapeutic applications. The kit includes a pre-coated 96-well microplate, lyophilised standard, sample and antibody dilution buffers, biotinylated detection antibody, HRP-Streptavidin conjugate, TMB substrate, stop solution, wash buffer, and plate sealers. Required materials not supplied include a microplate reader capable of measuring absorbance at 450 nm, pipettes and sterile tips, a 37°C incubator, and deionised or distilled water.