Human DEFA1 ELISA Kit

Designed for in vitro quantitative determination of human alpha Defensin 1, this Antibodies.com sandwich ELISA kit is suitable for serum, plasma, tissue homogenates, and other biological fluids. Reactivity is documented for human samples, with no reported cross-reactivity with other analogs or variants. The workflow uses a pre-coated microplate bearing a capture antibody specific to the target analyte.

The assay runs in approximately 4 hours and employs a biotinylated detection antibody, an HRP-Streptavidin conjugate, and a TMB substrate, with absorbance measured at 450 nm. Serum and plasma samples are recommended to be diluted at least 1:2 with Sample Dilution Buffer prior to testing; validated dilutions are 1:2 for healthy serum and 1:2 for healthy plasma. The reported analytical range is 31.25-2000 pg/mL with a sensitivity of <18.75 pg/mL.

Sample recovery in spiked matrices (n=10) is documented at 88%-100% (average 92%) for serum, 88%-92% (average 90%) for EDTA plasma, and 88%-104% (average 97%) for heparin plasma. Storage instructions specify shipping at +4°C with components stored at +4°C or -20°C as detailed in the protocol manual; freeze/thaw cycles should be avoided and the kit used before the stated expiration date.