Human Dedicator Of Cytokinesis 4 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com enables the in vitro quantitative determination of human Dedicator Of Cytokinesis 4 (DOCK4) in tissue homogenates or other biological fluids. The kit employs a pre-coated microplate with a colorimetric detection method, with absorbance read at 450 nm using a microplate reader.

Performance characteristics include a quantitative assay range of 0.156–10 ng/ml and a sensitivity of <0.063 ng/ml, with a total assay time of 4h 30m. Kit components are stored at +4°C or -20°C as instructed.

Validation data for serum, EDTA plasma, and heparin plasma samples are provided. Mean recovery values across five replicates were 91% for serum (range 80–102%), 90% for EDTA plasma (range 81–100%), and 84% for heparin plasma (range 80–89%). Dilution linearity across 1:2, 1:4, 1:8, and 1:16 dilutions was 87–91%, 87–107%, 74–101%, and 92–97% in serum; 90–105%, 84–101%, 90–101%, and 79–108% in EDTA plasma; and 84–95%, 92–105%, 82–105%, and 89–91% in heparin plasma, respectively.