Human Death Inducer Obliterator 1 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Death Inducer Obliterator 1 in tissue homogenates and other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, employing a biotinylated anti-target detection antibody followed by an HRP-avidin conjugate and TMB substrate. Results are measured as optical density at 450 nm on a colorimetric microplate reader.

The reported assay range is 0.312–20 ng/ml with a sensitivity of <0.104 ng/ml, and the total assay time is 4 hours 30 minutes. Kit components are stored at +4°C or -20°C as instructed. Linearity and recovery have been evaluated across serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, 1:8, and 1:16 (n=5 per sample type). Average recoveries were 91% in serum (range 80–102%), 90% in EDTA plasma (range 81–100%), and 84% in heparin plasma (range 80–89%).