Human D-Aspartate Oxidase ELISA Kit

A sandwich ELISA from Antibodies.com is intended for the in vitro quantitative determination of human D-Aspartate Oxidase (DDO) in tissue homogenates or other biological fluids. The format uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, a biotinylated anti-DDO detection antibody, and HRP-avidin with TMB substrate, producing a colourmetric read at 450 nm. The stated product range is 0.156–10 ng/ml and sensitivity is <0.059 ng/ml, with a total protocol time of 4 hours 30 minutes.

Reported sample recovery covers serum, EDTA plasma, and heparin plasma. Across the dilutions examined, the source provides an overall range plus an average value for each matrix: serum 80%–102% with an average of 91%; EDTA plasma 81%–100% with an average of 90%; heparin plasma 80%–89% with an average of 84%. Per-dilution recovery ranges are also reported at 1:2, 1:4, 1:8, and 1:16 for each sample type. Kit components include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, with storage at +4°C or -20°C as instructed.