Human Cytoplasmic Antiproteinase 3 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com is designed for the in vitro quantitative determination of human Cytoplasmic Antiproteinase 3 (also referred to as PI-9) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. The assay employs a pre-coated microplate format with colourmetric detection of HRP enzymatic activity via TMB substrate, read at 450 nm. The reported assay range is 1.56–100 ng/ml with a sensitivity of 0.45 ng/ml, and the total assay time is approximately 4 hours 30 minutes.

The kit is suitable for serum, plasma, cell lysates and tissue homogenates. Storage instructions specify keeping components at +4°C or -20°C as indicated, and the kit should not be used past its expiration date. Average recovery values reported across sample types include serum (n=5, 80–102%), EDTA plasma (n=5, 81–100%) and heparin plasma (n=5, 80–89%), with linearity-of-dilution data given for 1:2, 1:4, 1:8 and 1:16 dilutions across the same matrices.