Human Cytochrome C Oxidase Subunit I ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit enables in vitro quantitative determination of human MTCO1 (Cytochrome C Oxidase Subunit I) in tissue homogenates, cell lysates, and other biological fluids.

The assay uses a 96-well pre-coated microplate with a biotinylated anti-MTCO1 detection antibody, HRP-avidin conjugate, and TMB substrate, producing a colourmetric signal read at 450 nm. Reported performance includes an assay range of 0.312-20 ng/ml, a sensitivity of <0.119 ng/ml, and a total assay time of 4h 30m.

Validated recovery averages 91% in serum (range 80-102%), 90% in EDTA plasma (range 81-100%), and 84% in heparin plasma (range 80-89%), with dilution linearity demonstrated from 1:2 to 1:16 across all three matrices. Components are stored at +4°C or -20°C as instructed, and results are read on a colourmetric microplate reader.