Human Cystic Fibrosis Transmembrane Conductance Regulator ELISA Kit

Supplied by Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in tissue homogenates, cell lysates and other biological fluids. The assay uses a pre-coated microplate (12 x 8 well strips) and a colourimetric detection method read on a microplate reader at 450 nm, with a total assay time of 4 hours 30 minutes.

The reported detection range is 0.156–10 ng/ml and the sensitivity is 0.053 ng/ml. The procedure employs a biotinylated anti-CFTR antibody followed by an HRP-avidin conjugate, with TMB substrate and an acidic stop solution producing a yellow product whose absorbance is proportional to CFTR concentration.

Kit components include a pre-coated 96-well microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, intended for storage at +4°C or -20°C as instructed. Validated sample matrices include serum (80–102% recovery), EDTA plasma (81–100% recovery) and heparin plasma (80–89% recovery), with associated dilution linearity data.