Human Cyclin Dependent Kinase Inhibitor 2A ELISA Kit

A sandwich ELISA designed for the in vitro quantitative measurement of human Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) in tissue homogenates, cell lysates, and other biological fluids. The assay from Antibodies.com uses a pre-coated 96-well microplate arranged as 12 x 8 well strips and is read colorimetrically at 450 nm on a microplate reader.

The stated assay range is 0.625-40 ng/ml with a sensitivity of 0.234 ng/ml, and total assay time is 4 hours 30 minutes. The procedure employs a biotinylated anti-CDKN2A detection antibody, an HRP-avidin conjugate, and a TMB substrate; the resulting absorbance is proportional to CDKN2A concentration and is quantified against a standard curve.

Reported recovery values for serum, EDTA plasma, and heparin plasma average 91%, 94%, and 94% respectively across n=5 samples each, with linearity data provided across dilutions of 1:2 to 1:16. Kit components are stored at +4°C or -20°C as indicated.