Human CUB And Zona Pellucida Like Domains Protein 1 ELISA Kit

A sandwich ELISA from Antibodies.com for the in vitro quantitative determination of human CUB and Zona Pellucida Like Domains Protein 1 in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips), a biotinylated detection antibody, HRP-Avidin and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

According to the manufacturer, the measurable range is 0.312–20 ng/ml and the sensitivity is 0.114 ng/ml, with a total assay time of 4 hours 30 minutes. Reported average recovery values (n=5) are 91% for serum (range 80–102%), 90% for EDTA plasma (range 81–100%) and 84% for heparin plasma (range 80–89%).

Dilution linearity data supplied by the manufacturer for serum at dilutions of 1:2, 1:4, 1:8 and 1:16 (n=5) are 87–91%, 87–107%, 74–101% and 92–97%; for EDTA plasma 90–105%, 84–101%, 90–101% and 79–108%; and for heparin plasma 84–95%, 92–105%, 82–105% and 89–91%.