Human Crystallin Alpha B ELISA Kit

From Antibodies.com, this Human Crystallin Alpha B ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human alpha B Crystallin in tissue homogenates, cell lysates or other biological fluids [S1]. The assay employs the sandwich enzyme immunoassay technique on a pre-coated microplate to which standards and samples are added, with bound alpha B Crystallin detected using a Biotinylated Anti-alpha B Crystallin Antibody followed by HRP-Avidin conjugate and TMB substrate [S1]. The enzymatic reaction is visualised as a blue product that turns yellow on addition of acidic stop solution, and the density of yellow is proportional to the amount of alpha B Crystallin captured; absorbance is read at 450 nm in a colorimetric microplate reader and concentration calculated from the standard curve [S1].

The stated assay range is 0.625-40 ng/ml with a sensitivity of <0.216 ng/ml, and total assay time is 4h 30m [S1]. The manufacturer documentation reports recovery data across serum, EDTA plasma and heparin plasma matrices, with average recoveries of 91% for serum (range 80%-102%, n=5), 90% for EDTA plasma (range 81%-100%, n=5) and 84% for heparin plasma (range 80%-89%, n=5); linearity values are also provided across 1:2, 1:4, 1:8 and 1:16 dilutions for each of the three sample types [S1].

Components are stored at +4°C or -20°C as instructed and the kit should not be used past its expiration date [S1].