Human Cross Linked N-Telopeptide Of Type I Collagen ELISA Kit

Antibodies.com supplies this competitive ELISA kit for the quantitative determination of human Cross Linked N-Telopeptide Of Type I Collagen in serum, plasma, urine or other biological fluids. The assay is run on a pre-coated microplate and employs a biotinylated detection antibody with an HRP-Avidin reporter system and TMB substrate; the colour change is read at 450 nm on a colorimetric microplate reader. Total assay time is 2h 30m, and components are stored at +4°C or -20°C as instructed.

The stated product range is 3.12–200 ng/ml with a sensitivity of 0.86 ng/ml. Recovery was assessed using n=5 samples per matrix: serum 80%–102% (average 91%), EDTA plasma 81%–100% (average 90%), and heparin plasma 80%–89% (average 84%). Dilution linearity across four dilutions (1:2, 1:4, 1:8 and 1:16) with n=5 per matrix was reported as 87–91%, 87–107%, 74–101% and 92–97% for serum; 90–105%, 84–101%, 90–101% and 79–108% for EDTA plasma; and 84–95%, 92–105%, 82–105% and 89–91% for heparin plasma.