Human Cross Linked C-Telopeptide Of Type I Collagen ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit enables the in vitro quantitative determination of human Cross Linked C-Telopeptide Of Type I Collagen in serum, plasma or other biological fluids. An antibody specific to the target is pre-coated onto a 96-well microplate arranged as 12 x 8 well strips, and the assay is read on a colorimetric microplate reader at 450 nm. The stated product range is 0.156-10 ng/ml with a sensitivity of <0.059 ng/ml. Total assay time is 4h 30m. Kit components should be stored at +4°C or -20°C as instructed and not used past the expiration date.

Spike-and-recovery performance in serum averaged 91% (range 80%-102%, n=5), in EDTA plasma 90% (range 81%-100%, n=5), and in heparin plasma 84% (range 80%-89%, n=5). Dilution linearity was demonstrated across 1:2, 1:4, 1:8 and 1:16 sample dilutions for each matrix. The procedure uses a biotinylated detection antibody followed by an HRP-avidin conjugate and TMB substrate, with the absorbance of the yellow product being proportional to the analyte concentration in each well.