Human Coxsackie And Adenovirus Receptor Like Membrane Protein ELISA Kit

Manufactured by Antibodies.com, this Sandwich ELISA kit is designed for the in vitro quantitative determination of human Coxsackie And Adenovirus Receptor Like Membrane Protein in serum, plasma, tissue homogenates or other biological fluids. The assay employs the sandwich enzyme immunoassay technique, with a target-specific antibody pre-coated onto the wells of a microplate.

Standards and test samples are added to the wells, where the target present in each sample is bound by the immobilized antibody. After incubation and washing, a biotinylated detection antibody is added, which binds the captured target in each well. An HRP-avidin conjugate is then added, followed by further incubation and washing. TMB substrate solution is added; catalysis produces a blue product that turns yellow upon addition of acidic stop solution. The density of the yellow colour is proportional to the amount of target captured in the well. Absorbance is read at 450nm in a microplate reader, and concentration is calculated by reference to a standard curve.

The assay has a measuring range of 0.156-10 ng/ml with a sensitivity of <0.058 ng/ml and a total run time of 4h 30m. Kit components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.