Human Corticotropin Releasing Hormone Receptor 2 ELISA Kit

Manufactured by Antibodies.com, this is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) intended for the in vitro quantitative determination of human Corticotropin Releasing Hormone Receptor 2 (CRHR2) in tissue homogenates, cell lysates, or other biological fluids. The test uses a pre-coated microplate with an antibody specific for CRHR2, followed by a biotinylated anti-CRHR2 detection antibody and an HRP-Avidin conjugate. The enzymatic reaction is visualised using TMB substrate and read at 450 nm on a colorimetric microplate reader, with the resulting absorbance proportional to the CRHR2 concentration.

According to the manufacturer, the stated assay range is 0.156–10 ng/ml with a sensitivity of <0.051 ng/ml. Total assay time is 4 hours 30 minutes. Reported average recovery values are 91% for serum, 90% for EDTA plasma, and 84% for heparin plasma, with dilution linearity data reported across 1:2, 1:4, 1:8, and 1:16 dilutions for each matrix. Kit components are stored at +4°C or −20°C as indicated, and the kit is described as suitable for cell lysates and tissue homogenates.