Human Corticotropin Releasing Hormone Receptor 1 ELISA Kit

A sandwich-format quantitative immunoassay from Antibodies.com is designed for the in vitro measurement of human Corticotropin Releasing Hormone Receptor 1 (CRF1/CRHR1). The assay is reactive with human samples and employs a pre-coated 96-well microplate arranged as 12 x 8 well strips, with absorbance read at 450 nm on a colorimetric microplate reader. It is intended for use with tissue homogenates, cell lysates, or other biological fluids.

Sensitivity is reported as <0.060 ng/ml, with an assay range of 0.156–10 ng/ml and a total assay time of 4 hours 30 minutes. In the manufacturer's validation, average recovery was 91% for serum (range 80–102%, n=5), 90% for EDTA plasma (range 81–100%, n=5), and 84% for heparin plasma (range 80–89%, n=5). Kit components should be stored at +4°C or -20°C as instructed and the kit must not be used past its expiration date.