Human Copeptin ELISA Kit

A sandwich ELISA supplied by Antibodies.com for the quantitative determination of human Vasopressin in serum, plasma or other biological fluids. The Antibodies.com listing categorises the target as Copeptin, while the manufacturer protocol describes quantitative measurement of Vasopressin. The kit uses the sandwich enzyme immunoassay technique on a pre-coated 96-well microplate, with detection via an HRP-Avidin conjugate and TMB substrate, read at 450 nm in a colorimetric microplate reader. The manufacturer reports a sensitivity of 5.3 pg/ml and an assay range of 15.6-1000 pg/ml, with a total assay time of 4h 30m. Reported average recoveries are 91% for serum (range 80-102%), 90% for EDTA plasma (range 81-100%), and 84% for heparin plasma (range 80-89%), based on n=5 per sample type. Linearity upon dilution (1:2 to 1:16) was 74-107% across sample types. Reagent components are stored at +4°C or -20°C as instructed, and the product is for research use only.