Human Connective Tissue Growth Factor ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com enables the in vitro quantitative determination of human Connective Tissue Growth Factor (CTGF). The kit is intended for use with serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. A pre-coated 96-well microplate is provided, arranged as 12 x 8 well strips, with an antibody specific for CTGF immobilised on the plate surface.

Standards and test samples are added to the wells, where CTGF is captured by the immobilised antibody. After washing, a Biotinylated Anti-CTGF Antibody is added, followed by an HRP-Avidin conjugate. The enzymatic reaction is visualised with TMB substrate, producing a blue-coloured product that turns yellow upon addition of acidic stop solution. Absorbance is read at 450 nm on a microplate reader, and CTGF concentration is calculated by reference to the standard curve. The stated product range is 46.8–3000 pg/ml with a sensitivity of <17.5 pg/ml, and the total assay time is 4 h 30 min.

Reported average recovery is 91% in serum (range 80–102%, n=5), 90% in EDTA plasma (range 81–100%, n=5) and 84% in heparin plasma (range 80–89%, n=5). Kit components supplied include the pre-coated 96-well microplate, lyophilised standard, Detection Solution A, Detection Solution B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and adhesive plate sealers. Components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.