Human Colony Stimulating Factor 1, Macrophage ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com is designed for the in vitro quantitative determination of human Macrophage Colony-Stimulating Factor (M-CSF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The assay uses a 96-well microtiter plate pre-coated with an antibody specific for M-CSF, and detection is achieved with a Biotinylated Anti-M-CSF Antibody, an HRP-Avidin conjugate, and TMB substrate solution, with absorbance read at 450 nm on a colorimetric microplate reader.

Quantitative performance is supported by a manufacturer-stated assay range of 15.6–1000 pg/ml and a sensitivity of 6.5 pg/ml, with an assay time of 4 hours 30 minutes. Reported mean recovery (n=5) is 91% from serum (range 80–102%), 90% from EDTA plasma (range 81–100%), and 84% from heparin plasma (range 80–89%). Dilution linearity is provided across 1:2, 1:4, 1:8, and 1:16 dilutions for serum (87–91%, 87–107%, 74–101%, 92–97%), EDTA plasma (90–105%, 84–101%, 90–101%, 79–108%), and heparin plasma (84–95%, 92–105%, 82–105%, 89–91%).

Kit components include a pre-coated 96-well microplate (12 x 8 well strips), lyophilized standard, Detection Solution A, Detection Solution B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and plate sealers, with storage of components at +4°C or −20°C as instructed and use only before the stated expiration date.