Human Collagen Type XVII ELISA Kit

From Antibodies.com, this ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Collagen XVII in serum, plasma, tissue homogenates, or other biological fluids. The assay uses the sandwich enzyme immunoassay technique on a 96-well microtiter plate (12 x 8 well strips) pre-coated with an antibody specific for Collagen XVII.

Standards and samples are added to the wells, followed by a Biotinylated Anti-Collagen XVII Antibody and an HRP-Avidin conjugate. Detection is achieved using TMB substrate solution, with absorbance read at 450 nm in a microplate reader. The assay has a sensitivity of 1.35 ng/ml, a measuring range of 3.12-200 ng/ml, and a total run time of 4 hours 30 minutes.