Human Collagen Type XII ELISA Kit

From Antibodies.com, this sandwich ELISA kit enables the in vitro quantitative determination of human Collagen Type XII (COL12A1) in serum, plasma, tissue homogenates and other biological fluids. The assay uses a pre-coated microplate and the sandwich enzyme immunoassay technique, with biotinylated anti-COL12A1 antibody and HRP-avidin conjugate detection. TMB substrate produces a colourmetric signal measured at 450 nm in a microplate reader [S1].

Reported sensitivity is <0.128 ng/ml with an assay range of 0.312–20 ng/ml [S1]. Average recovery values are 91% for serum, 96% for EDTA plasma, and 95% for heparin plasma [S1]. Dilution linearity is documented across 1:2, 1:4, 1:8 and 1:16 dilutions for each sample type [S1]. Total assay time is 4 hours 30 minutes [S1].

Kit components include a pre-coated microplate, lyophilised standard, detection solutions A and B, wash buffer concentrate, sample dilution buffer, TMB substrate, stop solution and adhesive plate sealers, with storage temperatures varying between +4°C and -20°C depending on the component [S1].