Human Collagen Type XI Alpha 1 ELISA Kit

A sandwich ELISA from Antibodies.com for the in vitro quantitative determination of human Collagen Type XI Alpha 1 (COL11A1) in serum, plasma and other biological fluids. The procedure uses a pre-coated microplate, a biotinylated anti-COL11A1 detection antibody and an HRP-Avidin conjugate, with TMB substrate producing a colour change that is read at 450 nm. Sensitivity is 15 pg/ml and the standard curve range is 39–2500 pg/ml. Validation data are provided for serum, EDTA plasma and heparin plasma, including reported recovery ranges of 80–102% (serum), 81–101% (EDTA plasma) and 80–89% (heparin plasma), alongside linearity across 1:2 to 1:16 dilutions for the same matrices.

Total assay time is approximately 4 h 30 min. Reagent components are stored at +4°C or -20°C as instructed, and the product is intended for research use only.