Human Coiled Coil Domain Containing Protein 3 ELISA Kit

Manufactured for Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Coiled Coil Domain Containing Protein 3 (CCDC3) in serum, plasma, tissue homogenates, or other biological fluids. The assay uses a pre-coated microplate arranged as 12 x 8 well strips, with absorbance read at 450 nm on a colorimetric microplate reader.

Reported sensitivity is <0.126 ng/ml, with an assay range of 0.312–20 ng/ml. The complete procedure takes approximately 4 hours 30 minutes and employs HRP-Avidin conjugate detection with TMB substrate. Kit components include a lyophilized standard, Detection Solutions A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and adhesive plate sealers, stored at +4°C or −20°C as instructed.

Validation data show average recoveries of 91% for serum (range 80–102%), 90% for EDTA plasma (range 81–100%), and 84% for heparin plasma (range 80–89%) across five samples each, with linearity demonstrated across dilutions from 1:2 to 1:16 in all three matrices. The kit is intended for research use only and is not for diagnostic or therapeutic applications.