Human Cluster Of Differentiation 40 Ligand ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com intended for the in vitro quantitative determination of human Cluster of Differentiation 40 Ligand (also referred to as TRAP/CD40L). The assay is performed on a pre-coated 96-well microplate (12 x 8 well strips) using a biotinylated anti-TRAP/CD40L detection antibody, HRP-avidin conjugate, and TMB substrate. The colour change is read at 450 nm on a colorimetric microplate reader and the analyte concentration is calculated against a standard curve. The product is suitable for serum, plasma, tissue homogenates, cell lysates, or other biological fluids.

The stated product range is 0.156–10 ng/ml with a sensitivity of <0.051 ng/ml, and the assay is completed in 4 hours 30 minutes. Kit components are stored at +4°C or −20°C as instructed. Reported average recovery values are 91% (range 83–100%) for serum (n=5), 96% (range 91–102%) for EDTA plasma (n=5), and 95% (range 89–102%) for heparin plasma (n=5). Dilution linearity at 1:2, 1:4, 1:8, and 1:16 is reported across the same three sample types.

Supplied components include a pre-coated 96-well microplate, lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and adhesive plate sealers.