Human Clock Homolog ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (ELISA) is designed for the in vitro quantitative determination of human KAT13D/CLOCK in tissue homogenates, cell lysates, or other biological fluids. The assay uses a pre-coated microplate format, with the target captured by an immobilized specific antibody and detected using a biotinylated secondary antibody followed by HRP-Avidin and TMB substrate, producing a colour change measured at 450 nm.

The kit delivers a sensitivity of < 0.109 ng/ml and a quantitative range of 0.312–20 ng/ml, with a total run time of 4 hours 30 minutes. It is intended for use with a colorimetric microplate reader and is suitable for serum, EDTA plasma, and heparin plasma samples in addition to cell lysates and tissue homogenates. Reported recovery averages are 91% for serum (range 80–102%, n=5), 90% for EDTA plasma (range 81–100%, n=5), and 84% for heparin plasma (range 80–89%, n=5), with dilution linearity documented across 1:2 to 1:16 dilutions for each sample matrix.

Kit components include a pre-coated microplate, lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers. Components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.