Human Claudin 9 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Claudin 9 in tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) and employs a biotinylated anti-Claudin 9 detection antibody followed by HRP-avidin and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

The measuring range is 78.1–5000 pg/ml with a sensitivity of 33 pg/ml, and the total assay time is 4 hours 30 minutes. Kit components are stored at +4°C or -20°C as instructed, and components should not be used past their expiration date.

Validation data report recovery in serum (n=5) averaging 91% across an 80–102% range, EDTA plasma (n=5) averaging 90% across 81–100%, and heparin plasma (n=5) averaging 84% across 80–89%.