Human Claudin 1 ELISA Kit

The brand Antibodies.com offers a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Claudin 1 in tissue homogenates, cell lysates, and other biological fluids. The kit employs a pre-coated 96-well microtiter plate with an antibody specific for Claudin 1; standards and samples are added, then detected using a Biotinylated Anti-Claudin 1 Antibody and an HRP-Avidin conjugate with TMB substrate, reading absorbance at 450 nm against a standard curve.

Reported sensitivity is 0.059 ng/ml with an assay range of 0.156–10 ng/ml. The assay runs on a colorimetric microplate reader using pre-coated 12 x 8 well strips and completes in 4h 30m. Spike-and-recovery testing gave ranges of 80%–102% for serum (average 91%, n=5), 81%–100% for EDTA plasma (average 90%, n=5), and 80%–89% for heparin plasma (average 84%, n=5). Kit components are stored at +4°C or -20°C as instructed.