Human Chymotrypsinogen B1 ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the quantitative in vitro determination of human CTRB1 (Chymotrypsinogen B1) in serum, plasma, or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, and results are read colorimetrically at 450 nm.

The reported assay range is 1.56-100 ng/ml with a sensitivity of 0.68 ng/ml. The protocol employs a sandwich enzyme immunoassay workflow: standards and samples are added to coated wells, captured CTRB1 is detected using a biotinylated anti-CTRB1 antibody, and an HRP-avidin conjugate is visualised with TMB substrate, producing a blue product that turns yellow after addition of stop solution. The absorbance at 450 nm is proportional to CTRB1 concentration and is interpolated against a standard curve.

Recovery has been characterised in serum (n=5; range 80-102%, average 91%), EDTA plasma (n=5; 81-100%, 90%), and heparin plasma (n=5; 80-89%, 84%). Dilution linearity has been assessed for serum, EDTA plasma, and heparin plasma at 1:2, 1:4, 1:8, and 1:16 dilutions. Total assay time is 4h 30m, and kit components should be stored at +4°C or -20°C as instructed and not used past the indicated expiration date.