Human CD27 Binding Protein ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit from Antibodies.com, catalogued under Assays and Kits and designed for the in vitro quantitative determination of human SIVA (catalogue target designation: CD27 Binding Protein) in tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips): standards and samples are added, the target is captured by the immobilised antibody, and detection is carried out using a biotinylated anti-SIVA antibody, HRP-avidin conjugate, and TMB substrate. The colour change is read at 450 nm on a colorimetric microplate reader, and the target concentration is calculated from the standard curve.

Manufacturer-reported performance specifications include an assay range of 0.156–10 ng/ml, a sensitivity of <0.068 ng/ml, and a total assay time of 4 h 30 min. Average recovery is reported as 91% from serum (n=5, range 80–102%), 91% from EDTA plasma (n=5, range 81–101%), and 84% from heparin plasma (n=5, range 80–89%). Kit components are stored at +4°C or −20°C as instructed, and the kit must not be used past its expiration date.