Human CCL7 ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) is designed for the in vitro quantitative determination of human MCP3 in serum, plasma, tissue homogenates, and other biological fluids. The kit is specific for MCP3, with no reported cross-reactivity with other analogs or variants.

It uses a pre-coated microplate in a sandwich format with a biotinylated detection antibody and an HRP-Streptavidin conjugate, with TMB substrate read at 450 nm on a colorimetric microplate reader. The stated product range is 15.625-1000 pg/mL with a sensitivity of <9.375 pg/mL, and the total assay time is 4 hours.

Reported recovery (average) was 98% in serum (range 87% - 105%, n=10), 98% in EDTA plasma (range 91% - 103%, n=10), and 90% in heparin plasma (range 85% - 95%, n=10). Components are shipped at +4°C and stored at +4°C or -20°C as instructed in the protocol manual.