Human CCAAT/Enhancer Binding Protein Beta ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human CEBP beta in tissue homogenates, cell lysates, or other biological fluids. The assay uses a pre-coated microplate and employs the sandwich enzyme immunoassay technique, with a biotinylated anti-CEBP beta antibody and an HRP-avidin conjugate. Detection is visualised with TMB substrate, producing a blue colour that converts to yellow upon addition of stop solution; absorbance is read at 450 nm using a colorimetric microplate reader. The total assay time is 4h 30m.

Reported sensitivity is 0.061 ng/ml, with an assay range of 0.156–10 ng/ml. The kit is supplied with a pre-coated microplate (12 x 8 well strips), lyophilised standard, detection reagents A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, intended for storage at +4°C or -20°C as instructed. Reported average recovery is 91% for serum (range 80–102%), 90% for EDTA plasma (range 81–100%), and 84% for heparin plasma (range 80–89%), with linearity data provided across dilutions from 1:2 to 1:16.