Human Caudal Type Homeobox Transcription Factor 2 ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of human Caudal Type Homeobox Transcription Factor 2 (CDX2) in tissue homogenates, cell lysates, serum, plasma, and other biological fluids [S1, S3]. The assay uses a CDX2-specific antibody pre-coated onto a 96-well microplate arranged as 12 x 8 well strips [S1, S3]. Standards and samples are added to the wells, followed by a Biotinylated Anti-CDX2 Antibody, an HRP-Avidin conjugate, and TMB substrate, with the resulting colour change read at 450 nm on a colorimetric microplate reader [S1, S3]. The total assay time is 4 hours 30 minutes [S1].

The kit has a reported sensitivity of 0.063 ng/ml and a working range of 0.156–10 ng/ml [S1, S3]. Reported spike-and-recovery values include averages of 91% in serum (range 80%–102%, n=5), 90% in EDTA plasma (range 81%–100%, n=5), and 84% in heparin plasma (range 80%–89%, n=5) [S1]. Linearity has been evaluated across 1:2, 1:4, 1:8, and 1:16 sample dilutions in the same three matrices [S1]. Kit components are stored at +4°C or -20°C, and the product is intended for research use only [S1, S3].