Human CASP8 And FADD Like Apoptosis Regulator ELISA Kit

An in vitro quantitative sandwich enzyme-linked immunosorbent assay (sELISA) from Antibodies.com designed to measure human FLIP (CASP8 And FADD Like Apoptosis Regulator) in tissue homogenates, cell lysates or other biological fluids. The kit employs a pre-coated 96-well microplate and a sandwich immunoassay workflow in which standards and samples are incubated with an immobilised capture antibody, followed by a Biotinylated Anti-FLIP detection antibody and an HRP-Avidin conjugate. A TMB substrate produces a coloured product whose absorbance is read at 450 nm using a colorimetric microplate reader.

Manufacturer-stated performance includes a sensitivity of 0.061 ng/ml and an assay range of 0.156–10 ng/ml, with a total assay time of 4h 30m. Spike-and-recovery values are reported for serum (80%–102%, average 91%, n=5), EDTA plasma (81%–100%, average 90%, n=5) and heparin plasma (80%–89%, average 84%, n=5), and linearity has been assessed across dilutions of 1:2, 1:4, 1:8 and 1:16 in those same matrices. Kit components should be stored at +4°C or −20°C as instructed and not used past the expiration date.