Human Cartilage Oligomeric Matrix Protein ELISA Kit

This Sandwich ELISA kit from Antibodies.com enables in vitro quantitative determination of human COMP/Cartilage Oligomeric Matrix Protein in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, or other biological fluids. The assay employs a sandwich enzyme immunoassay technique on a pre-coated 96-well microplate formatted as 12 x 8 well strips, with absorbance read at 450 nm in a colorimetric microplate reader.

The stated product range is 62.5–4000 pg/ml, with a sensitivity of 23.9 pg/ml. Reported recovery averages are 91% for serum (range 80–102%, n=5), 90% for EDTA plasma (range 81–100%, n=5), and 84% for Heparin plasma (range 80–89%, n=5), alongside dilution linearity data from 1:2 to 1:16 across each sample type. Total assay time is 4h 30m. Kit components include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, stored at +4°C or –20°C as instructed.