Human Canopy 2 Homolog ELISA Kit

From Antibodies.com, this Sandwich ELISA kit is designed for the in vitro quantitative determination of human CNPY2/MSAP (Canopy 2 Homolog) in tissue homogenates, cell lysates, and other biological fluids. It uses a pre-coated microplate in a 12 x 8 well strip format and is read on a colorimetric microplate reader at 450 nm.

The assay employs a biotinylated anti-CNPY2/MSAP detection antibody followed by an HRP-avidin conjugate, with TMB substrate producing a yellow product whose absorbance is proportional to the amount of CNPY2/MSAP captured in each well. The reported sensitivity is <0.062 ng/ml, the assay range is 0.156–10 ng/ml, and the total assay time is 4h 30m.

Manufacturer-reported average spike-and-recovery values are 91% in serum, 90% in EDTA plasma, and 84% in heparin plasma. Kit components include a pre-coated microplate, lyophilized standard, detection reagents, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, stored at +4°C or -20°C as indicated on individual items.