Human calprotectin ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) supplied by Antibodies.com for the in vitro quantitative determination of human Calprotectin in serum, plasma, tissue homogenates, and other biological fluids. The 4-hour assay uses a pre-coated microplate to which standards and samples are added; Calprotectin is captured by an immobilised antibody, then detected with a biotinylated antibody that forms an antibody–antigen–antibody sandwich. Bound complex is visualised with an HRP-Streptavidin conjugate and TMB substrate, producing a blue product that turns yellow on addition of stop solution, with absorbance read at 450 nm.

The kit is specific for Calprotectin with no reported cross-reactivity with other analogs or variants. Performance characteristics include a working range of 0.625–40 ng/mL and a sensitivity below 0.375 ng/mL, read on a colorimetric microplate reader. Validation studies reported average recoveries for undiluted serum, EDTA plasma, and heparin plasma of 96%, 94%, and 93% respectively (n=10). Recommended working dilutions were 1:200–1:1000 for healthy serum and EDTA plasma (n=50 each), with linearity demonstrated across 1:2, 1:4, and 1:8 dilutions for all three sample types. Serum and plasma samples should be diluted at least 1:2 with Sample Dilution Buffer prior to testing.

Kit components include a pre-coated 96-well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody, antibody dilution buffer, HRP-Streptavidin conjugate, SABC dilution buffer, TMB substrate, stop solution, wash buffer, plate sealers, and a foil zip-sealed pouch. The kit is shipped at +4°C with components stored at +4°C; freeze/thaw cycles should be avoided and the kit must not be used past its expiration date.