Human Calpain 3 ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Calpain 3 in tissue homogenates or other biological fluids. The assay uses a pre-coated microplate in a 12 x 8 well strip configuration and is read on a colorimetric microplate reader at 450 nm. The kit's stated sensitivity is 0.125 ng/ml and the assay range is 0.312–20 ng/ml, with a total assay time of 4h 30m. Kit components include a pre-coated microplate, lyophilized standard, Detection Solution A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and adhesive plate sealers; components are stored at +4°C or –20°C as instructed.

Reported recovery for serum samples (n=5) ranged from 80–102% (average 91%); EDTA plasma (n=5) showed 81–100% recovery (average 90%); and heparin plasma (n=5) showed 80–89% recovery (average 84%). Linearity was assessed at dilutions of 1:2, 1:4, 1:8, and 1:16 across serum, EDTA plasma, and heparin plasma, with reported ranges varying by sample type. The product is intended for research use only and is not for diagnostic or therapeutic applications.