Human Caldesmon ELISA Kit

From Antibodies.com, this sandwich ELISA is designed for the in vitro quantitative determination of human Caldesmon/CDM in tissue homogenates, cell lysates, or other biological fluids. The assay employs a pre-coated 96-well microplate configured as 12 x 8 well strips, read on a colorimetric microplate reader at 450 nm, with a total run time of 4 hours 30 minutes.

Performance specifications from the manufacturer include an assay range of 0.781–50 ng/ml and a sensitivity of <0.25 ng/ml. Detection relies on a biotinylated anti-Caldesmon/CDM antibody and HRP-avidin conjugate, with TMB substrate producing a colour change proportional to analyte captured in each well.

Reported recovery averages are 91% for serum (range 80–102%), 90% for EDTA plasma (range 81–100%), and 84% for heparin plasma (range 80–89%), each at n=5. Dilution linearity has been evaluated at 1:2, 1:4, 1:8, and 1:16 across the same sample types. Kit components are stored at +4°C or −20°C as instructed.