Human Calcium/Calmodulin Dependent Protein Kinase Kinase 2 ELISA Kit

Manufactured by Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) enables in vitro quantitative determination of human CAMKK2 in tissue homogenates or other biological fluids. The kit uses a 96-well microplate pre-coated with an antibody specific for CAMKK2, with detection carried out using a Biotinylated Anti-CAMKK2 Antibody, HRP-Avidin conjugate and TMB substrate; absorbance is read at 450nm on a colorimetric microplate reader. Reactivity: Human.

Reported sensitivity is 0.114 ng/ml, with a product range of 0.312–20 ng/ml and an assay time of 4 hours 30 minutes. Kit components are stored at +4°C or -20°C as instructed. Validation across serum, EDTA plasma and heparin plasma samples (n=5) shows average recoveries of 91% (serum, range 80–102%), 91% (EDTA plasma, range 81–101%) and 84% (heparin plasma, range 80–89%), with linear dilution recovery assessed at 1:2, 1:4, 1:8 and 1:16.