Human C-Myc Binding Protein ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) is designed for the in vitro quantitative determination of human AMY-1 (C-Myc Binding Protein) in tissue homogenates or other biological fluids [S1][C0]. The assay uses a 96-well microtiter plate pre-coated with an antibody specific for the target. Standards and test samples are added to the wells, and following incubation and washing, a biotinylated anti-target antibody is introduced, followed by an HRP-avidin conjugate. TMB substrate is then used to visualise the enzymatic reaction, producing a blue product that turns yellow upon addition of acidic stop solution. Absorbance is read at 450 nm in a microplate reader and target concentration is calculated by reference to a standard curve [S1].

According to the manufacturer, the assay has a sensitivity of <0.061 ng/ml and a working range of 0.156-10 ng/ml, with a total assay time of 4 hours 30 minutes [S1]. The kit is intended for use with a colorimetric microplate reader, and components should be stored at +4°C or -20°C as instructed; the kit should not be used past its expiration date [S1].

Manufacturer validation data report average spike-and-recovery values of 91% in serum (n=5, 80-102%), 90% in EDTA plasma (n=5, 81-100%) and 84% in heparin plasma (n=5, 80-89%). Dilution linearity has been assessed at 1:2, 1:4, 1:8 and 1:16 in each sample matrix, with the reported recovery ranges being: serum 87-91%, 87-107%, 74-101%, 92-97%; EDTA plasma 90-105%, 84-101%, 90-101%, 79-108%; heparin plasma 84-95%, 92-105%, 82-105%, 89-91% [S1].